Fig. 7

PKC inhibitor chelerythrine pretreatment rescues paclitaxel-induced deficits in neuronal morphology. (A) Representative traces of granule cells in the dentate gyrus, scale bar shown is 50 μm. (B-C) Paclitaxel treatment reduced the complexity and length of granule cells of the dentate gyrus, which could be rescued by chelerythrine pretreatment. (B) Repeated measures two-way ANOVA, Distance: F(3.451, 400.3) = 174.6, p < 0.0001; Treatment: F(3, 116) = 5.600, p = 0.0013; Distance x Treatment: F(60, 2320) = 2.413, p < 0.0001. Dunnet’s multiple comparisons test between Chel/Veh and Chel/PTX: p < 0.05 between 90 and 160 μm from the soma. (C) One-way ANOVA: followed by Tukey post-hoc test: p < 0.0001 for Veh/Veh vs. Veh/PTX, p = 0.001 for Veh/PTX vs. Chel/PTX, p = 0.98 for Veh/Veh vs. Chel/PTX. (D) Representative traces of cortical neurons, basal dendrites are colored grey, apical dendrites are colored black, scale bar shown is 100 μm. (E-G) Paclitaxel treatment reduced the complexity and length of apical dendrites of layers 2/3 cortical pyramidal neurons in the medial prefrontal cortex, which could be rescued by chelerythrine pretreatment. (E) Distance: F(3.961, 459.5) = 457.9, p < 0.0001; Treatment: F(3, 116) = 0.9192, p = 0.43; Distance x Treatment: F(45, 1740) = 1.366, p = 0.055. (F) One-way ANOVA, p = 0.41. (G) Distance: F(6.408, 743.4) = 152.9, p < 0.0001; Treatment: F(3, 116) = 5.895, p = 0.0009; Distance x Treatment: F(75, 2900) = 1.587, p = 0.0011. p < 0.05 at 50, 80, and 160 μm from the soma. (F) p = 0.0003 for Veh/Veh vs. Veh/PTX, p = 0.007 for Veh/PTX vs. Chel/PTX, p = 0.97 for Veh/Veh vs. Chel/PTX. N = 6 neurons per mouse, 5 mice per group