Fig. 5
Neuronal and glial localization of LAMP1- and SAP-C-labelled lysosomes surrounding the Aβ plaque area. A Fixed brain samples from 1.5-, 6-, 8-, and 12-month (m)-old APPNL-G-F mice were co-stained with LAMP1 and IBA1, SAMI31, or GFAP antibodies. At 1.5 m and 6 m, LAMP1+-DNs were co-labeled with SMI31, but in older mouse brains (12 m), LAMP1 largely co-localized with IBA1. B Fixed brain sections from 1.5-, 6-, and 9-m-old APPNL-G-F mice were co-stained with SAP-C and neurofilament L (NFL) antibodies, and brain sections from 1.5-, 3-, 6-, 9-, 15 and 21-m-old APPNL-G-F mice were triple-stained with SAP-C, IBA1, and GFAP. SAP-C was largely co-localized with NFL at swelled dystrophic axons, but it did not co-label with IBA1, even at older ages. However, strong labeling of SAP-C in astrocytes in the plaque vicinity, as labeled by GFAP, was evident in older mouse brains