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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer’s disease brains

Fig. 6

LAMP1-labeled phagocytic DAM surrounding plaque in AD brains. A Brain sections from 4- and 15-month (m)-old APPNL-G-F mice were co-stained with IBA1 and LPL or sections from 15-m-old APPNL-G-F mice were co-stained with LAMP1 and LPL. B Fixed brain sections from 15-m-old APPNL-G-F mice were triple-stained with LAMP1, IBA1, and LPL. C AD postmortem brain sections were triple-stained with Thio-S, IBA1, and LAMP1. D Fixed and processed blocks of brains from 10-m-old 5xFAD mice were subjected to 3D electron microscopy (3D EM). Microglia (blue dashed line), dense bodies (turquoise dashed line), and DNs (green dashed line) in close proximity to an amyloid plaque (orange dashed line) are shown on a single micrograph from 3D-EM image stacks. Filopodia-like morphology in microglia engulfing DNs is evident in the image. Amyloid plaque (orange), microglia (blue), dense bodies inside microglia (turquoise), and DNs (green) were tracked through a stack of ~ 100 images and a 3D structure of the whole plaque was reconstructed using Reconstruct software

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