Fig. 8

Alteration of SAP-C and cathepsin-D protein levels in lysosomes or autolysosomes in AD mouse brains. A A schematic diagram showing the variable size of lysosomes, autophagosome and mitochondria enriched in the fractionation experiment. Fusion of a lysosomes and autophagosome during autophagy-lysosomal degradation produce larger autolysosome organelles. Variable sized organelles were separated in mitochondria - and lysosomes-enriched fractions (MFs or LFs) by sequential centrifugation. The final supernatant is designated as cytosolic fractions (CFs). B Proteins in MFs, LFs and CFs were analyzed on the Western blot with the indicated antibodies. C Band intensity for each protein was measured and normalized by the GAPDH level (N = 3 independent experiments, * P < 0.05, ** P < 0.01, student t-test). SAP-C protein level was increased, but mature cathepsin-D level was reduced in autolysosomes in 5xFAD mouse brains