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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: SETD7-mediated monomethylation is enriched on soluble Tau in Alzheimer’s disease

Fig. 3

Nuclear, low-molecular weight Tau is preferentially methylated. A Cortical tissue from 30 week old rTg4510 mice was subjected to subcellular fractionation and the distribution of Tau was analyzed by Western blotting. The purity of fractions was determined by analyzing the distribution of marker proteins. As demonstrated by a representative blot, the soluble nuclear fraction contains abundant Tau protein but is free from the cytosolic proteins Hsp90, β3-Tubulin and Calnexin. B Representative Western blots and quantification demonstrate that meK130 and meK132-modified Tau preferentially localizes to the soluble nuclear fraction in the cortex of 30 week-old rTg4510 mice. C Triton-X soluble and insoluble fractions from hippocampi of 30 week-old rTg4510 mice were analyzed by Western blotting. For the distribution between the soluble and the pellet fraction, both HMW and LMW bands were quantified. While pT231-modified Tau is more abundant in the pellet and the HMW fraction, meK132-modified Tau is evenly distributed between soluble fraction and pellet and more abundant on LMW Tau. Statistical significance in (B) and (C) was determined by paired two-tailed Student’s t-test. *: p < 0.05, **: p < 0.01

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