Fig. 5
From: SETD7-mediated monomethylation is enriched on soluble Tau in Alzheimer’s disease
The SETD7 inhibitor (R)-PFI-2 reduces Tau methylation at K130 and K132. A Representative Western blots and quantification demonstrate a significant decrease in meK132-modified Tau after treatment of SHSY5Y cells with 5 μM (R)-PFI-2 for 24 h. Total Tau levels remain unaltered. B Electrochemiluminescence ELISA shows that treatment with 5 μM (R)-PFI-2 for 24 h reduces the levels of meK130-modified Tau in SH-SY5Y cells. Total Tau levels remain unaltered. C Western blots and quantification demonstrate a significant decrease in meK132-modified Tau after treatment of hiPSC-derived neurons with 5 μM (R)-PFI-2 for 48 h. Total Tau levels remain unaltered. D Electrochemiluminescence ELISA shows that treatment with 5 μM (R)-PFI-2 for 48 h reduces the levels of meK130 Tau in iPS-derived neurons. Total Tau levels remain unaltered. Statistical significance for all panels was determined by Mann Whitney test. **: p < 0.01, ***: p < 0.001