Fig. 1

Progranulin can be digested by multiple lysosomal proteases in vitro. a, Schematic representation of progranulin (PGRN) protein. Black arrows indicate inter-granulin linkers, individual granulins named granulins A-G (~ 7 kD each) and paragranulin (p) (~ 3.5 kD) are annotated. b-d, 1 μM of each enzyme was incubated with 400 ng of recombinant human PGRN at the indicated pH for 20 min. The proteins were silver stained (shown in greyscale) to assess the presence or absence of PGRN at every condition. All assays were repeated n = 3 times