Fig. 2

Plaque-associated human microglia accumulate Lipid Droplets, and Lipid Droplet area is reduced in TREM2-R47H transplanted mice. A Lipid droplets (red, PLIN2) accumulate in human microglia (green, GFP) adjacent to plaques in chimeric mice (blue, Amylo-Glo), but not in microglia distant from plaques;B with a nearly identical relationship observed in human microglia (green, IBA1) in AD postmortem brains; C Quantification of PLIN2 in GFP TREM2-R47H vs. GFP TREM2 xMGs (green, GFP; red, PLIN2) surrounding amyloid plaques (blue, Amylo-glo) in 7-month old 5X-hCSF1 reveal (D) a significant reduction in total PLIN2 area in R47H xMG transplanted mice (E) with no significant differences in total plaque area, F a significant reduction of the percentage of PLIN2 area normalized to plaque area was also detected in R47H xMG transplanted mice; G with no significant difference in the number of PLIN2⁺GFP xMGs in vicinity to plaques; Scale Bars at 10 mm in A, 5 mm in B, and 20 mm in C, n=3-4; 5-6 images per mouse; * P< 0.05; ** P < 0.01