Fig. 3

xMGs expressing TREM2 upregulate activation markers around plaques, but less so in TREM2-R47H xMGs. A Quantification of xMG proximity to amyloid plaques (blue, Amylo-glo) in 7-month old 5X-hCSF1 revealed a reduction of plaque-associated GFP TREM2-R47H xMGs vs. GFP TREM2 xMGs (green, GFP; red, CD9), C within 10 mm per plaque; D A significant reduction in total levels of CD9, E without a significant difference in total plaque load, and F a significant reduction of the percentage of CD9 area normalized to plaque area was observed in TREM2-R47H vs. TREM2 xMGs. B, G PLIN2 (red) accumulating GFP xMGs (green) express the DAM-specific marker CD9 (purple) near plaques (blue, Amylo-Glo); H GFP TREM2-R47H vs. TREM2 xMGs (green, GFP; Red, APOE; white, LAMP1) reveal a significant reduction in total APOE area (I), with no significant difference in total plaque load (J), and a significant reduction in APOE area normalized to plaque area (K). An increased trend of the percentage of LAMP1 normalized to plaque area (L) was observed in TREM2-R47H xMGs without reaching significance (P=0.5635). Scale Bars at 10 mm in A and 20 mm in H; * P < 0.05; ** P < 0.01; **** P < 0.0001; n=3-4 mice per genotype; 5-6 images per mouse; Confocal images B(scales at 5 mm); n=3-4 mice per genotype; 3-4 images per mouse