Skip to main content
Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Functional microRNA targetome undergoes degeneration-induced shift in the retina

Fig. 1

Global retinal mRNA expression profile determined by high-throughput sequencing (HTS). (A) Retinas from mice subjected to 5 days photo-oxidative damage (PD; n = 4), or dim-reared controls (dim; n = 4), were collected and RNA extracted. Sequencing libraries were prepared and subjected to HTS Illumina sequencing. Obtained reads were cleaned and trimmed and aligned to the mm10 mouse genome. (B) Principal Component Analysis (PCA) demonstrated tight clustering of the dim and PD groups with the exception of dim sample 1. (C) Hierarchical clustering was performed with for the top 80 differentially expressed mRNA and illustrated with a heat map. Sample specific expression level of each mRNA is indicated by the z-score color scale. (D) Gene set enrichment analysis (GSEA) for all differentially expressed mRNAs was performed against the C2 database with down-regulated pathways indicated in red and up-regulated pathways in blue (P < 0.05)

Back to article page