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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Functional microRNA targetome undergoes degeneration-induced shift in the retina

Fig. 2

Global retinal miRNA expression profile determined by high-throughput sequencing (HTS). (A) Retinas from mice subjected to 5 days photo-oxidative damage (PD; n = 4), or dim-reared controls (dim; n = 4), were collected and small RNA extracted. Sequencing libraries were prepared and subjected to HTS Illumina sequencing. Obtained reads were cleaned and trimmed and aligned to mature mouse miRNA sequences (miRBase). (B) PCA analysis demonstrated good clustering of samples inter-group. (C) Hierarchical clustering was performed with for the top 80 differentially expressed miRNA and illustrated with a heat map. Sample specific expression level of each miRNA is indicated by the z-score color scale. (D) Hierarchical clustering was also performed for the top 20 most highly-expressed miRNAs with significant miRNA color coded on the left-hand panel (P < 0.05). (E-F) Pie charts illustrating the expression level of the global retinal miRNA in the dim (E) and PD (F) samples

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