Fig. 6

Enriched sequence motifs of AGO2-bound mRNA. Peak calling (HOMER) of reads mapped to AGO2-bound mRNAs was performed for DR and PR samples. Peaks were annotated according to transcript region and subjected to motif enrichment analysis (HOMER). The most highly enriched motif for each sample is shown here. For easy orientation, the cDNA sequence of miR-124-3p is displayed at the top, with positions 1–6 indicated by the dashed box and positions 2–8 indicated by the solid box. (A) The top most enriched binding motifs of AGO2-bound mRNAs in dim retinas was the exact sequence of the miR-124-3p seed region. (B) The most enriched binding motifs of AGO2-bound mRNAs in PD retinas are the reverse complement of positions 2–8 (or 1–8 for the 8-mer motifs) of the miR-124-3p binding regions encompassing all canonical seed regions as indicated with the colored lines