Fig. 1

Cross-species comparison of MC gliosis in response to laser injury. (A-E) Analysis of MC gliotic response in zebrafish and mice at baseline (Uninjured) and different time point after injury (Day 1, 3, 7 and 14). Detection of GFAP in GS⁺MCs after laser induction in zebrafish (A.i-A.v) and mice (B.i-B.v). Shown are sections for GS (red) and GFAP (green). Detection of phospho-Erk1/2 in GS⁺MCs after injury and in uninjured zebrafish (C.i-C.v) and mice (D.i-D.v). Shown are sections for GS (red) and phospho-Erk1/2 (green). Histograms illustrating mean ± SD of GS⁺, GFAP⁺ and pERK1/2⁺cells normalized by the total of DAPI⁺ or GS⁺ cells in percentage (G). Significant differences (***p < 0.001, ****p < 0.0001) between uninjured and injured retinas were determined by post-hoc Bonferroni one-way ANOVA test (n = 12). (E-F; H) Evaluation of the proliferative potential of zebrafish and murine MCs during injury response at baseline (Uninjured) and different time point after injury (Day 1, 3, 7 and 14). Detection of PCNA in GS⁺MCs after injury in zebrafish (E.i-E.v) and mice (F.i-F.v). Shown are sections for GS (red) and PCNA (green). Histograms illustrating mean ± SD of PCNA⁺cells normalized by the total of GS⁺cells in percentage (H). Significant differences (****p < 0.0001) between uninjured and injured retinas were determined by post-hoc Bonferroni one-way ANOVA test (n = 12)