Fig. 3

Difference of MC injury response in zebrafish and mice. (A, B) Heatmaps of differentially expressed genes (DEGs) associated with an epithelial phenotype in sorted cycling zebrafish and murine MCs. The blue box groups the most significant genes with the highest upregulation. (C-H) Analysis of MC phenotype in zebrafish and mice at the baseline (Uninjured) and at different time points after injury (Day 1, 3, and 7). Detection of E-cadherin in GS+ MCs after laser induction in zebrafish (C.i-C.iii) and mice (D.i-D.iii). Shown are retinal sections stained for GS (red) and E-cadherin (green). Zoomed-in view of murine GS+/E-cadherin+ cells of the area defined by a blue frame at Day 7 (d.i-d.iv). White arrowheads mark double-positive cells. (E) Histograms illustrating the mean ± SD of the number of E-cadherin+ cells normalized by the total number of GS+ cells expressed in percentage. Significant differences (****p < 0.0001) between uninjured and injured retinas were determined by using a post-hoc Bonferroni one-way ANOVA test (n = 12). Detection of N-Cadherin in GS+ MCs after laser induction in zebrafish (F.i-F.iii) and mice (G.i-G.iii). Shown are retinal sections stained for GS (red) and N-Cadherin (green). Zoomed-in view of zebrafish GS+/N-Cadherin+ cells of the area defined by a blue frame at Day 3 and 7 (f.ii1-f.ii4, f.iii1-f.iii4). Zoomed-in view of murine GS+/N-Cadherin+ cells of the area defined by a blue frame at Day 1 (g.i-g.iv). White arrowheads mark double-positive cells. (H) Histograms illustrating the mean ± SD of the number of N-Cadherin+ cells normalized by the total number of GS+ cells expressed in percentage. Significant differences (****p < 0.0001) between uninjured and injured retinas were determined by using a post-hoc Bonferroni one-way ANOVA test (n = 12)