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Fig. 10 | Molecular Neurodegeneration

Fig. 10

From: METTL3-dependent RNA m6A dysregulation contributes to neurodegeneration in Alzheimer’s disease through aberrant cell cycle events

Fig. 10

Inhibition of m6A demethylation reduces shMettl3-induced deficits in primary neurons. Primary cortical neurons were infected by AAV-GFP-shRNA at around DIV7. About 8–9 days after infection, primary neurons were used for analysis. Representative immunofluorescence image (A) and quantitative analysis of m6A (B) in the primary cortical neurons after AAV-shRNAs infection with/without co-treatment of 0.25 μM rhein (inhibitor for m6A demethylation) for 24 h. (C-I) Representative immunoblot (C) and quantitative analysis of cleaved caspase 3 (D), CCND2 (E) and CCND1 (F) in primary neurons after AAV-shRNAs infection with/without co-treatment of 0.25 μM rhein. (n = 3 in each group). Representative immunofluorescence image (G) and quantitative analysis of PSD95 (H) and MAP 2 (I) in the neurites of primary cortical neurons after AAV-shRNAs infection with/without co-treatment of Rhein. (J) Cell death was measured by propidium iodide (PI) uptake in primary cortical neurons after AAV-shRNA infection with/without co-treatment of Rhein (Data are means±SEM from at least 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001; B, D-F, H-J one-way ANOVA with bonferroni’s correction)

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