Fig. 11

Manipulated METTL3 expression impacts AβO-induced PSD95 loss in primary neurons. (A-B) Representative immunoblot (A) and quantitative analysis (B) of METTL3 protein expression in primary rat cortical neurons treated by 2.5 μM AβOs at DIV14 for 3 days. (C-H) Primary rat cortical neurons were infected by AAV-shMettl3 (C, D, G) or AAV-METTL3 (E, F, H) at ~DIV7 and challenged by 2.5 μM AβOs at DIV14 for 3 days. Representative immunoblot (C, E) and quantification analysis (D, F) of METTL3 and PSD95 at DIV17–18 were shown. Representative immunofluorescence images of PSD95 in neurites were shown (G, I) and relative PSD95 immunoreactivity was quantified (H, J). (Data are means±SEM from at least 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001; B, unpaired student’s t-test; D, F, H, J one-way ANOVA with bonferroni’s correction)