Fig. 5

L-DL deficiency impairs U2 spliceosome dependent RNA splicing. (a-b) Molecular function (a) and biological process (b) terms in GO analysis for L-DL associated proteins identified in mass spectrometry. c Protein-protein interaction (PPI) network for L-DL associated proteins (PPI enrichment P < 1.0E-16). The network clustering is based on k-mean clustering and each cluster is labelled with a distinct colour. d A drawing of U2 spliceosome assembly on pre-mRNAs. 5′SS or 3′SS indicate 5′ or 3′ splicing sites, respectively. e CTRL-GST or GST-L-DL were introduced into 293T cells, followed by pull-down with GST agarose beads. L-DL associated proteins were determined by immunoblotting. f GST-L-DL was introduced into 293T cells, followed by immunoprecipitation with anti-U2AF65 antibody. Levels of U2AF65 associated proteins were measured by immunoblotting. g, h Control or L-DL KO N2a cell lysates were subjected to immunoprecipitation with anti-U2AF65 antibody. Levels of U2AF65 associated proteins were measured by immunoblotting (g) and densitometric analyses (n = 3) (h). Statistical analysis was performed using two-tailed Student’s t-test; * P < 0.05, ** *** P < 0.001; error bars denote SEM