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Table 2 List of antibodies used with dilutions and conditions

From: Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model

Immunocytochemistry /Immunofluorescence
1°Antibody (1°Ab) Dilution Specificity Host Antigen Retrieval
81A 1:1000 αSyn at pSer129 Mouse Water boil w/ 0.05% Tween
2H6 1:5000 αSyn (2–21) Mouse Water boil w/ 0.05% Tween
EP1536Y 1:1000 αSyn at pSer129 Rabbit Water boil w/ 0.05% Tween
5G4 1:1000 oligomeric, aggregated αSyn (44–57) Mouse Water boil w/ Citrate/70% Formic acid
AB5 1:1000 Mouse 70% Formic acid/Water boil w/ Citrate
12F4 1:500 1–42 Mouse 70% Formic acid/Water boil w/ Citrate
33.1.1 1:500 1–16 Mouse 70% Formic acid/Water boil w/ Citrate
13.1.1 1:800 1–40 Mouse 70% Formic acid/Water boil w/ Citrate
GFAP (from Dako) 1:2000 Astrocytes, glial cells Rabbit Water boil w/ 0.05% Tween
Iba1 1:1000 Macrophages, microglia Rabbit Water boil w/ Citrate/70% Formic acid
p62 1:2000 Sequestresome1 Rabbit Water boil w/ 0.05% Tween
NFL 1:500 NFL Rabbit Water boil w/ Citrate
Western blotting
1°Antibody (1°Ab) Dilution Epitope Host
94-3A10 1:1000 human and mouse αSyn (130–140) Mouse
6E10 1:1000 Mouse
15-4A5 1:1000 human αSyn (120–125) Mouse
C4 1:1000 actin Mouse