From: Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model
Immunocytochemistry /Immunofluorescence | ||||
1°Antibody (1°Ab) | Dilution | Specificity | Host | Antigen Retrieval |
81A | 1:1000 | αSyn at pSer129 | Mouse | Water boil w/ 0.05% Tween |
2H6 | 1:5000 | αSyn (2–21) | Mouse | Water boil w/ 0.05% Tween |
EP1536Y | 1:1000 | αSyn at pSer129 | Rabbit | Water boil w/ 0.05% Tween |
5G4 | 1:1000 | oligomeric, aggregated αSyn (44–57) | Mouse | Water boil w/ Citrate/70% Formic acid |
AB5 | 1:1000 | Aβ | Mouse | 70% Formic acid/Water boil w/ Citrate |
12F4 | 1:500 | Aβ1–42 | Mouse | 70% Formic acid/Water boil w/ Citrate |
33.1.1 | 1:500 | Aβ1–16 | Mouse | 70% Formic acid/Water boil w/ Citrate |
13.1.1 | 1:800 | Aβ1–40 | Mouse | 70% Formic acid/Water boil w/ Citrate |
GFAP (from Dako) | 1:2000 | Astrocytes, glial cells | Rabbit | Water boil w/ 0.05% Tween |
Iba1 | 1:1000 | Macrophages, microglia | Rabbit | Water boil w/ Citrate/70% Formic acid |
p62 | 1:2000 | Sequestresome1 | Rabbit | Water boil w/ 0.05% Tween |
NFL | 1:500 | NFL | Rabbit | Water boil w/ Citrate |
Western blotting | ||||
1°Antibody (1°Ab) | Dilution | Epitope | Host | |
94-3A10 | 1:1000 | human and mouse αSyn (130–140) | Mouse | |
6E10 | 1:1000 | Aβ | Mouse | |
15-4A5 | 1:1000 | human αSyn (120–125) | Mouse | |
C4 | 1:1000 | actin | Mouse |