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Table 2 List of antibodies used with dilutions and conditions

From: Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model

Immunocytochemistry /Immunofluorescence

1°Antibody (1°Ab)

Dilution

Specificity

Host

Antigen Retrieval

81A

1:1000

αSyn at pSer129

Mouse

Water boil w/ 0.05% Tween

2H6

1:5000

αSyn (2–21)

Mouse

Water boil w/ 0.05% Tween

EP1536Y

1:1000

αSyn at pSer129

Rabbit

Water boil w/ 0.05% Tween

5G4

1:1000

oligomeric, aggregated αSyn (44–57)

Mouse

Water boil w/ Citrate/70% Formic acid

AB5

1:1000

Mouse

70% Formic acid/Water boil w/ Citrate

12F4

1:500

1–42

Mouse

70% Formic acid/Water boil w/ Citrate

33.1.1

1:500

1–16

Mouse

70% Formic acid/Water boil w/ Citrate

13.1.1

1:800

1–40

Mouse

70% Formic acid/Water boil w/ Citrate

GFAP (from Dako)

1:2000

Astrocytes, glial cells

Rabbit

Water boil w/ 0.05% Tween

Iba1

1:1000

Macrophages, microglia

Rabbit

Water boil w/ Citrate/70% Formic acid

p62

1:2000

Sequestresome1

Rabbit

Water boil w/ 0.05% Tween

NFL

1:500

NFL

Rabbit

Water boil w/ Citrate

Western blotting

1°Antibody (1°Ab)

Dilution

Epitope

Host

94-3A10

1:1000

human and mouse αSyn (130–140)

Mouse

6E10

1:1000

Mouse

15-4A5

1:1000

human αSyn (120–125)

Mouse

C4

1:1000

actin

Mouse