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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Human iPSC-derived astrocytes transplanted into the mouse brain undergo morphological changes in response to amyloid-β plaques

Fig. 2

hiPSC-astrocytes integrate functionally within the mouse brain. (a-b) A xenografted hiPSC-astrocyte in the chimeric mouse brain (a, red) and a GFAP+ cortical astrocyte in the human brain (b, brown) contacting blood vessels with their end-feet. Scale bars: 25 μm. (c) hiPSC-astrocyte processes (RFP, red) express the gap junction marker Cx43 (green, arrows). Scale bar: 2 μm. (d) The gap-junction dye Alexa488 loaded on a hiPSC-astrocyte (RFP+, red) diffuses into RFP- neighboring host cells. Scale bar: 25 μm. (e-h) Enlarged views of the area selected in d. (e) RFP+ hiPSC-astrocyte, (f) Alexa488 dye, (g) Nuclei stained with DAPI, (h) Overlay. Arrows point to Alexa488+ RFP- host cells. (i-k) Representative traces of current injection steps of 20mV (i), resting membrane potentials (j) and current-voltage (I/V) curves (k) of hiPSC-astrocytes in the host brain (n = 17 cells from 6 WT mice). Data are represented as mean ± SEM

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Molecular Neurodegeneration

ISSN: 1750-1326