Fig. 4

Four subtypes of morphologically defined GFAP+ astrocytes in the human entorhinal cortex and white matter. (a) Overview of human entorhinal cortex layers stained with GFAP (brown) to detect astrocytes. Layers molecular to lamina dissecans are mainly composed of subpial interlaminar astrocytes, while layers pri-α to pri-γ are rich in protoplasmic astrocytes (arrows). (b-f) Representative images of subpial interlaminar astrocytes (b) and their tortuous processes (c), protoplasmic astrocytes (d), varicose-projection astrocytes (e) and their beaded processes (f). (g-i) Human white matter GFAP+ fibrous astrocytes (h-i). mol: molecular layer, diss: lamina dissecans. Scale bars: 50 μm in (a) and (g); 25 μm in (b) and (h); 10 μm in (c-f) and (i)