Fig. 5

Human astrocytes go through morphological transformations becoming hypertrophic or atrophic in response to Aβ plaques within the chimeric AD brain. (a-h, a’-h’) hiPSC-astrocytes (RFP+, white or red) exposed to Aβ plaques (Thioflavin, green) show quiescent (a-c, a’-c’), hypertrophic (d-f, d’-f’) and atrophic (g-h, g’-h’) morphologies in chimeric AD mice five months after transplantation. Scale bars: 25 μm. (i) Percentage of hiPSC-astrocytes remaining quiescent, or becoming hypertrophic or atrophic as a group (n = 13 mice). Data are represented as mean ± SEM, one-way ANOVA with Friedman test, ***p < 0.001. (j) Percentage of hiPSC-astrocytes remaining quiescent, or becoming hypertrophic or atrophic per APOE genotype (n = 6 mice for APOE E3/E3; n = 7 mice for APOE E4/E4) five months post-transplantation. Data are represented as mean ± SEM, Chi-square test: n.s., non-significant