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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Adult-onset CNS myelin sulfatide deficiency is sufficient to cause Alzheimer’s disease-like neuroinflammation and cognitive impairment

Fig. 3

CNS sulfatide depletion induces a chronic immune/inflammatory response characterized by strong progressive activation of microglia and astrocytes. (A) Venn diagram showing the number of specific and shared differentially expressed genes (DEGs) in the CRM and SC of CST cKO mice compared to their respective controls from the Nanostring AD panel. Shared DEGs are listed. (B) Gene Ontology (GO) analysis for the 16 shared DEGs was performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID). Top functions are listed. (C) Venn diagram showing the number of DEGs found in at least three groups from six groups of different CNS regions/time points from both CST cKO and CST KO mice. Heatmap displaying log2 fold changes of the 72 DEGs that were shared by both CST cKO and CST KO mice. DEGs were grouped into three major categories by lines of different color and ordered based on how many times they were significantly altered in six groups. See Fig. S3F for the list of the DEGs that were specific to CST cKO or CST KO mice. Heteroscedastic Welch’s t-Test, n = 3–4. p-values are displayed within each heat map cell (*p < 0.05). (D) List of the top functions from GO analysis for the 72 shared DEGs. (E)) Western blot analysis from cerebrum and spinal cord of 4.5 mo and 9 mo post-injection CST cKO mice using antibodies against Iba1 and GFAP. Relative expression was quantified and plotted as a ratio to GAPDH. Two-tailed unpaired t-Test, n = 4. *p < 0.05, **p < 0.01. Data represent the mean ± S.E.M

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