Fig. 5

Adult-onset myelin sulfatide deficiency lead to marked astrogliosis and microgliosis within specific myelin-enriched brain regions. (A) MALDI-imaging of an adult WT mouse brain displaying the distribution of the most abundant sulfatide species (ST(N24:1), m/z 888, green), as well as a neuronal rich phosphatidylinositol species (PI(38:5), m/z 883.5, red). (B-E) Representative immunofluorescence images of CST cKO Cre⁻ and CST cKO Cre⁺ mice 9 mo post-injection using antibodies against GFAP (red) and Iba1 (green) on brain (B,C) and brain stem (D,E). Scale bar: 500 μm (B, C) and 200 μm (D,E). Quantification of GFAP IF staining area percentage on (cortex + corpus callosum) and brain stem was shown in (G). (F) Co-staining of GFAP (red) and myelin basic protein (MBP, green) in brain of CST cKO Cre⁻ and CST cKO Cre⁺ mice 9 mo post-injection. Scale bar: 500 μm. (G) GFAP relative expression on (cortex + corpus callosum) and brain stem was quantified and plotted as a IF staining area percentage. (H) Electron microscopy images from the spinal cord of CST cKO Cre⁻ and CST cKO Cre⁺ mice 11 mo post-injection. Astrocytes, identified by the intermediate filament GFAP and glycogen clusters were highlighted by the yellow dash line. Cx: cortex (Cx1: outer cortex without enriched sulfatide; Cx2: inner cortex with enriched sulfatide); CC: corpus callosum; CA1: Cornu Ammonis 1 region of the hippocampus; DG: hippocampal dentate gyrus