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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories

Fig. 3

Western blot analyses of αSyn_RT-QuIC products from OM-seeded reactions. Representative Western blot images showing the presence of PK resistant bands in αSyn_RT-QuIC end products generated by PD (n = 8) (a) and MSA-P (n = 8) samples (b). Conversely, no bands were detected in αSyn_RT-QuIC end products seeded with HS (n = 8) (c) and MSA-C (n = 8) (d). Statistical analysis revealed that PD generated products were significantly less resistant to PK digestion compared to those generated by MSA-P (Mann-Whitney test p = 0.0104). Densitometric analyses have been performed on Western blot images of αSyn_RT-QuIC products generated from PD (n = 13), MSA-P (n = 20), MSA-C (n = 10) and HS (n = 11) OM samples at ITA-lab (see Supplementary Fig. 3a). Median with interquartile range is shown (e)

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Molecular Neurodegeneration

ISSN: 1750-1326