Fig. 3

MiRNAs miR-672-5p, let-7 g-5p, miR-100-5p, and miR-298-5p induce cytokine and chemokine release from microglia. Microglia isolated from C57BL/6 mice were incubated with 5 μg/ml of candidate miRNA, as indicated, for either 24 h (Gro-α, IFN-γ, IL-6, MIP-2) or 36 h (other cytokines/chemokines). The TLR7 agonist loxoribine (1 mM) and the TLR4 agonist LPS (100 ng/ml) served as positive control. Unstimulated cells were used as negative control. The release of depicted inflammatory mediators was subsequently analyzed by multiplex immune assay. Data are shown as fluorescent intensity (FI) values expressed as a. u., arbitrary units, mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 compared to negative control, one-way ANOVA with Holm-Sidak’s multiple comparison test