Fig. 6
Cre-dependent anterograde monosynaptic tracing of H129-dgK-G4. A. Schema of the simplified lateral septal nucleus (LS) projection pathways. LS, lateral septal nucleus; CA1, field CA1 of hippocampus; CA3, field CA3 of hippocampus; MeA, medial amygdaloid nucleus, anterior part. B. The tracing timeline. Helper virus AAV2/9-DIO-mCh-gK (1.0 × 1012 vg/ml, 100 nl) and H129-dgK-G4 (5.0 × 108 pfu/ml, 100 nl) were sequentially injected into the same region of LS in GAD2-Cre transgenic mice at day 1 and day 22, respectively. The brains were collected at day 27 and images were obtained after cryosection. C-F. Representative tracing results. The representative images of the injection site of LS (C) and the downstream projection target brain regions (D–F) are shown. The boxed areas are presented in the right panels with higher magnification, and the starter neurons labeled by both GFP and mCherry are indicated with white arrows. Representative images shown are of the same brain from 3 mice. The right hemisphere displays the corresponding sections from Allen Brain Reference Atlases (Image credit: Allen Institute) (D–F)