Skip to main content
Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Edaravone activates the GDNF/RET neurotrophic signaling pathway and protects mRNA-induced motor neurons from iPS cells

Fig. 2

mRNA-induced MN generation from iPSCs. (A) Diagram of the differentiation protocol. (B) Brightfield (BF) microscopic images show iPSC1 cells and differentiated cells at indicated days (Bar: 100 μm). Cells at day 4 and 7 of differentiation were immunostained for TUJ1 for quantification (Bar: 20 μm; DAPI: nuclei). (C) iPSCs and ESCs (105 cells) were differentiated by mRNAs. TUJ1+ cells at differentiation day 4 (before passaging) and 7 (after replating) were counted after TUJ1 immunostaining with cell numbers shown inside each panel. Data represents Mean ± SEM (n = 6, 3 independent differentiation, and each differentiation provides 2 wells of cells for immunostaining and quantification)

Back to article page

Molecular Neurodegeneration

ISSN: 1750-1326