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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Edaravone activates the GDNF/RET neurotrophic signaling pathway and protects mRNA-induced motor neurons from iPS cells

Fig. 3

miMNs express pan-neuronal and MN lineage markers. (A and B) HB9+/TUJ1+, Islet1+/TUJ1+ and ChAT+/TUJ1+ neurons were detected by immunostaining and quantified in miMNs from the iPSC1, iPSC2 and ESC line. Neurons at day 10 (HB9 and Islet1) and 20 (ChAT) of differentiation were analyzed. (C) HB9+/ChAT+/TUJ1+ and Islet1+/ChAT+/TUJ1+ cells were quantified in miMNs from the iPSC1 line (day 20 of differentiation). (D and E) iPSC1-derived miMNs (day 30 of differentiation) were immunostained for the mature neuron marker Synapsin-1, glutamatergic marker VGlut1 and GABAergic marker GAD67. Cell nuclei were counterstained with DAPI (Bar = 20 μm). Data represents Mean ± SEM (n = 6, 3 independent differentiation, and each differentiation provides 2 wells of cells for immunostaining and quantification)

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Molecular Neurodegeneration

ISSN: 1750-1326