Fig. 6

Transcriptomic analysis of edaravone-induced responses in miMNs. (A) Volcano plot showing DE genes after edaravone treatment in control miMNs from iPSC1 (red and blue dots: genes with FDR ≤ 0.01 and log₂(fold change) ≥ 0.5 or ≤ − 0.5, respectively). (B) Top ten IPA pathways enriched in up- and down-regulated genes. Pathways are ranked based on -log(p value) as calculated by the Fisher’s exact test. (C) IPA pathways of interest and their associated genes. Genes selected for qPCR validation are highlighted. (D and E) Control and ALS miMNs were treated with edaravone (10 μM, 24 h) or DMSO as the vehicle control. Total cellular RNAs were subjected to qPCR analysis of up-regulated (left panel) and down-regulated (right panel) genes highlighted in C. Data represents Mean ± SEM from 3 technical replicates (*: p < 0.05, t test with the Bonferroni correction for multiple comparisons)