Fig. 2

Analysis of PGRN and granulin levels in brain regions and spinal cord. a Western blot analysis of primary cortical neuron (DIV14) and microglia lysates using sheep anti-PGRN antibodies. n = 4. Data presented as mean ± SEM. *, p < 0.05, unpaired two-tailed Student’s t-test. b Western blot analysis of tissue lysates from 4.5 to 5 months old male WT mice with antibodies against full-length of PGRN and individual granulin A and C as indicated. CX = cortex, Hp = hippocampus, CC = corpus callosum, Tha = thalamus, Cb = cerebellum, BS = brain stem, SC = spinal cord. c Quantification of experiment in (b). The ratio of each granulin peptide to full-length PGRN was quantified and normalized to the value of spinal cord samples on the same gel (set as 1). n = 3–5. Data presented as mean ± SEM. *, p < 0.05, **, p < 0.01, ***, p < 0.001, unpaired two-tailed Student’s t-test