Fig. 2

Aβ interacts with MRV in vitro and limits infection in vivo. A Interactions between purified MRV stock incubated for 2 h with PBS, Tris-nAu control, monomeric Aβ₄₂ (mAβ₄₂), oligomeric Aβ₄₂ (oAβ₄₂), or fibrillar Aβ₄₂ (fAβ₄₂) labeled with nanogold. Blue arrowhead: Viral particle. Red arrowhead: nanogold⁺ labeling. Green arrowhead: Fibrillar Aβ. A representative image with scale bar = 50 nm shown for each image with 5X increased magnification inset (white box represents area of inset) in the bottom left of image for viral particles or right of image for fibril. B Quantification of viral particles positive for nanogold (at least 50 particles were scored for each condition). C, D, Purified MRV stock incubated with PBS (MRV control) or oligomeric Aβ₄₂ (MRV Aβ₄₂) for 2 h, then i.p. injected into P0 BALB/c mice. Thymus was collected 7 dpi and evaluated for absolute number of CD4⁺, CD8⁺, DP (CD4⁺CD8⁺ double positive) or DN (CD4⁻CD8⁻ double negative) C, or MRV DNA load as copies/mL in the thymus D. ø represents below levels of detection. Data expressed as mean ± SD, one-way ANOVA with Tukey’s post hoc test (two-sided). *P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. No other statistical comparisons are significant unless indicated