Fig. 3

Peripheral MRV infection in adult 5XFAD mice does not affect Aβ plaque burden. A Schematic of experimental paradigm. B Absolute number of CD3⁺, CD4⁺, or CD8⁺ T cells in the spleen of 6-months-old 5XFAD mice infected with mock or MRV (i.p.). C Copies/mL of MRV DNA in cortex at 6-months-old or 7 dpi in thymus. D, E, Representative images of HJ3.4B staining for pan-Aβ immunoreactivity D and quantification of percent area coverage in the cortex E. Scale bar: 500 μm. F–I, Protein concentrations of PBS-soluble Aβ₄₀ (f) and Aβ₄₂ (g), or 5 M guanidine-soluble (“insoluble”) Aβ₄₀ H and Aβ₄₂ I in the cortex. J–L, Representative images of Iba1 staining for microglia J and quantification of percent area coverage in cortex K and hippocampus L. Scale bar: 500 μm. ø represents below levels of detection. Data expressed as mean ± SEM , –, multiple t-tests using Holm-Sidak method B, one-way ANOVA with Tukey’s post hoc test (two-sided) C. *P < 0.05. No statistical comparisons are significant unless indicated