Skip to main content
Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Murine roseolovirus does not accelerate amyloid-β pathology and human roseoloviruses are not over-represented in Alzheimer disease brains

Fig. 4

CNS MRV infection in adult 5XFAD does not accelerate Aβ formation but induces neuroinflammation. A Schematic of experimental paradigm. B Copies of MRV ORF69 DNA 72 hpi of mock or MRV, delivered bilaterally into the hippocampus of 6-week-old 5XFAD mice. C–E, Representative images of HJ3.4B staining for pan-Aβ immunoreactivity (Aβ-IR) with insets depicting intracellular Aβ-IR C and quantification of percent area coverage in the cortex D or hippocampus E. F Quantification of extracellular Aβ plaque deposits in cortex overlaying hippocampus. G–H, CNS inflammatory response to MRV in the hippocampus measured by gene transcript changes G and Iba1+ immunostaining for microglia (H, I). hpi: hours post injection. Scale bar: 500 μm. Data expressed as mean ± SEM, student’s t-test (b, d, e, f, h), and multiple t-tests F. *P < 0.05, ** P < 0.01. ns = not statistically significant. No statistical comparisons are significant unless indicated

Back to article page