Study | PMID | Platform | Study design | Note |
---|---|---|---|---|
Grubman et al. 2019 [41] | 31768052 | 10x isolated single-nuclei RNA sequencing | 6 AD, 6 controls from tissue repository | |
Mathys et al. 2019 [8] | 31042697 | 10x isolated single-nuclei RNA sequencing | 24 AD pathology, 24 no pathology from tissue repository | |
Alsema et al. 2020 [25] | 33192286 | 10x single-cell RNA sequencing and Smart-seq2 | FACS sorted microglia from 27 autopsy samples within 6 h after death | 10x scRNA-seq and Smart-seq2 showed some differences, mainly small clusters suspectedly due to plate-based protocols in Smart-seq2. |
Lau et al. 2020 [42] | 32989152 | 10x isolated single-nuclei RNA sequencing | 12 AD and 8 control from tissue repository | Male and female ratio were balanced in AD and control |
Nguyen et al. 2020 [43] | 32840654 | 10x isolated single-nuclei RNA sequencing | 15 AD from tissue repository | Samples were selected with varying APOE genotypes and pathologies, but matched for age and sex. |
Gerrits et al. 2021 [44] | 33609158 | 10x isolated single-nuclei RNA sequencing | 10 AD | 10 AD samples representing Braak stages 0, 2 and 6, all APOE ε3/ε3 genotypes |
Morabito et al. 2021 [45] | 34239132 | 10x isolated single-nuclei RNA sequencing and ATAC sequencing | 12 AD prefrontal cortex (PFC), 8 control PFC | |
Olah et al. 2020 [7] | 33257666 | 10x single-cell RNA sequencing | FACS sorted microglia from 10 AD, 4 Mild Cognitive Impairment and 3 temporal lobe epilepsy samples in DLPFC |