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Table 1 Summary of study design and single-cell RNA sequencing platforms in various human cohort studies of AD. PMID: PubMed id

From: Guidelines for bioinformatics of single-cell sequencing data analysis in Alzheimer’s disease: review, recommendation, implementation and application

Study

PMID

Platform

Study design

Note

Grubman et al. 2019 [41]

31768052

10x isolated single-nuclei RNA sequencing

6 AD, 6 controls from tissue repository

 

Mathys et al. 2019 [8]

31042697

10x isolated single-nuclei RNA sequencing

24 AD pathology, 24 no pathology from tissue repository

 

Alsema et al. 2020 [25]

33192286

10x single-cell RNA sequencing and Smart-seq2

FACS sorted microglia from 27 autopsy samples within 6 h after death

10x scRNA-seq and Smart-seq2 showed some differences, mainly small clusters suspectedly due to plate-based protocols in Smart-seq2.

Lau et al. 2020 [42]

32989152

10x isolated single-nuclei RNA sequencing

12 AD and 8 control from tissue repository

Male and female ratio were balanced in AD and control

Nguyen et al. 2020 [43]

32840654

10x isolated single-nuclei RNA sequencing

15 AD from tissue repository

Samples were selected with varying APOE genotypes and pathologies, but matched for age and sex.

Gerrits et al. 2021 [44]

33609158

10x isolated single-nuclei RNA sequencing

10 AD

10 AD samples representing Braak stages 0, 2 and 6, all APOE ε3/ε3 genotypes

Morabito et al. 2021 [45]

34239132

10x isolated single-nuclei RNA sequencing and ATAC sequencing

12 AD prefrontal cortex (PFC), 8 control PFC

 

Olah et al. 2020 [7]

33257666

10x single-cell RNA sequencing

FACS sorted microglia from 10 AD, 4 Mild Cognitive Impairment and 3 temporal lobe epilepsy samples in DLPFC