Fig. 4
From: Alzheimer risk gene product Pyk2 suppresses tau phosphorylation and phenotypic effects of tauopathy
Pyk2 expression is protective against Tau pathology in PS190/+ mice. A, Representative immunofluorescent images of DAPI, pTau S202/T205 (AT8) and pTau S199/S202 immunoreactivity in amygdala of 9.5–10.5-month-old WT, Pyk2−/−, PS190/+ and PS190/+;Pyk2−/− animals. Scale bar, 50 μm. B–D, Quantification of amygdalar pTau S202/T205 (AT8) immunoreactivity reveals significant increases in the number of AT8-positive cell-bodies (objects) (B) as well as in the area occupied by those objects (C) in PS190/+;Pyk2−/− compared to PS190/+ animals. Data are graphed as mean ± SEM, unpaired two-tailed t-test, *p < 0.05, n = 14–16 mice. E–G, Quantification of amygdalar pTau S199/S202 immunoreactivity reveals a significant increase in pTau S199/S202 mean image intensity (G) in PS190/+;Pyk2−/− compared to PS190/+ animals. Data are graphed as mean ± SEM, unpaired two-tailed t-test, *p < 0.05, n = 15–18 mice