Fig. 6
From: Alzheimer risk gene product Pyk2 suppresses tau phosphorylation and phenotypic effects of tauopathy
Proteomic analysis reveals signs of disrupted protein translational, increased synaptic C1q expression and decreased MAPK1 activity in PS190/+;Pyk2−/− animals. A–D, Synaptosomal fractions were prepared from hippocampi of 9.5–10.5-month-old PS190/+ and PS190/+;Pyk2−/− mice and run through LC-MS/MS to identify significantly differentially regulated proteins between PS190/+ and PS190/+;Pyk2−/− animals. A, Heat map showing relative abundance of significantly differentially regulated (p < 0.05) synaptic proteins between PS190/+ and PS190/+;Pyk2−/− animals. B, Volcano plot of all total synaptic proteins identified via LC-MS/MS. Positive values for Log2FC represent increased synaptic protein expression in PS190/+;Pyk2−/− compared to PS190/+ mice. Dashed line represents p = 0.05. Significantly differentially regulated synaptic proteins shown in red. C, Heat map showing relative abundance of significantly differentially regulated (p < 0.05), phospho-enriched, synaptic proteins (normalized to total protein abundance) between PS190/+ and PS190/+;Pyk2−/− animals. D, Volcano plot of all normalized, phospho-enriched, synaptic proteins identified via LC-MS/MS. Positive values for Log2FC represent protein upregulation in Pyk2−/− compared to WT. Dashed line represents p = 0.05