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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: VCP suppresses proteopathic seeding in neurons

Fig. 6

VCP knock down enhances αS seeding in neurons. A Immunofluorescence for phospho-αS and Tuj1 in HNs infected with lentiviral vectors expressingscrambled or one of two different shRNAs targeting VCP for 5 days and then treated with αS PFF or monomer (1μg/ml) for 5 days. Scale bar =20μm. B Quantitation of phospho-αS/Tuj1 staining in 3F. Experiments were repeated from 3 different cultures. N=7,5 and 5 for scramble and VCP shRNA 1 and 2 respectively. **p < 0.01 one-way ANOVA with Dunnett correction. Error bars are ± S.E.M. C Representative immunoblot from independent HN lysates treated with scramble or VCP shRNAs for anti-VCP and GAPDH. D Quantitation of band intensities of VCP over GAPDH control

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Molecular Neurodegeneration

ISSN: 1750-1326