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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: VCP suppresses proteopathic seeding in neurons

Fig. 7

VCP cofactor UBXD1 knockdown augments αS seeding. A αS biosensors were treated with scrambled control siRNA or siRNAs targeting the indicated genes for 48 hours prior to the application of αS PFF or monomer (10nM) and harvested for FRET efficiency at 24 hours. n =4 and 12 for the monomer and PFF treated group respectively for each KD. Adjusted p ****p < 0.0001 for UBXD1 by two-way ANOVA with Šidák correction; n.s.=no significance. Error bars are± S.E.M. B Immunofluorescence for phospho-αS and Tuj1 in HNs infected with lentiviral vectors expressing scrambled or a shRNA targeting UBXD1 for 5 days and then treated with αS PFF or monomer (1μg/ml) for 5 days Scale bar =20μm. C Quantitation of phospho-αS/Tuj1 staining from PFF treated HNs infected with scramble or control UBXD1 shRNAs. Experiments were repeated from 3 different cultures. N= 5 for each groups. * p<0.05 by Student’s t test). D Representative immunoblot from HN lysates treated with scramble or UBXD1 shRNAs for anti-UBXD1 and α-tubulin

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