Fig. 9

VCP disease mutations enhance αS seeding in vivo. A Drawing of coronal sections through mouse brains. Shaded regions indicate areas utilized for quantitation and boxes denote regions corresponding to the represntative images in B and D. B Representative immunofluorescence images with pSer129-syn antibody (red) of anterior cortices from C57, cVCP-R155C, and VCPR155H/WT mice injected unilaterally into the striatum with 5ug αS PFF after 90 days. 4 month aged C57 (n=10), VCP R155H/WT (n=5) and VCP R155C/FL; Rosa26-Cre ERT2 (cVCP-R155C) (n=5). Scale bar=25 μm. C Quantitation of the percentage of p-syn in entire anterior cortices (* P<0.05 by student’s t-test). D Representative immunofluorescence images with pSer129-syn antibody of posterior cortices from C57, cVCP-R155C, and VCPR155H/WT mice as described in A. scale bar=25 μm. (E) Quantitation of the percentage of p-syn in posterior cortices (* P<0.05, **P <0.01(* P<0.05 by student’s t-test). F-G Quantitation of p-syn in Amygdala and substantia Nigra. (ns, no significance student’s t-test)