Fig. 4

Impaired uptake of fAβ and lysosomal acidification in 6 month-old 5xFAD;Cx3cr1^−/− mice. (A) Representative flow cytometry plots identifying subpopulations of methoxy-X04⁺CD11b⁺CD45^low and methoxy-X04⁺CD11b⁺CD45^high microglia and (B) mean fluorescence intensities (MFI) for Lysotracker Deep-Red™ (-DR) to assess the acidification of endolytic compartments of these individual subpopulations. Data representative of flow cytometry experiments done using n = 5–6 mice (males and females) of 6 month-old 5xFAD;Cx3cr1^+/+ and 5xFAD;Cx3cr1^−/− mice. Quantification of (C) proportion of methoxy-X04⁺CD11b⁺CD45^low and methoxy-X04⁺CD11b⁺CD45^high microglia and (D) Lysotracker-DR MFIs for methoxy-X04^low and methoxy-X04^high microglia within the CD11b⁺CD45^low and CD11b⁺CD45^high subsets in 6 month-old 5xFAD;Cx3cr1^+/+ (black bars) and 5xFAD;Cx3cr1.^−/− (grey bars) mice. Data in C,D represents means calculated using n = 5–6 mice (males and females) per genotype. Error bars represent SEM. All animals were processed and analyzed in a single experiment to enable MFI comparisons across genotypes and individual mice. Statistics for data in C,D was done using two-way ANOVA with Tukey’s multiple comparison tests. *p < 0.05, **p < 0.001, ***p < 0.0001, ****p < 0.00001