Fig. 5

Early accumulation of larger foci of severe neuritic dystrophy in 4 month-old 5xFAD;Cx3cr1^−/− mice. Characterization of cortical dystrophic neurites (DNs) in 4 month-old 5xFAD mice with and without Cx3cr1 using α-LAMP1, α-Ubiquitin and α-nT-APP antibodies. Quantification of (A) LAMP-1⁺ and (B) Ubiquitin⁺ DNs in 5xFAD;Cx3cr1^+/+ (black bars) and 5xFAD;Cx3cr1^−/− (grey bars) mice. Error bars represent SEM. Statistical analysis done using two-tailed, standard Student t test with Welch’s correction for unequal SDs. ** p < 0.005 (C) nT-APP⁺ neurites (grey) associated with (solid arrows) compact vs. (dashed arrows) diffuse ThioS⁺ plaques (green) in the cortex of 4 month-old (top panels) 5xFAD;Cx3cr1^+/+ and (bottom panels) 5xFAD;Cx3cr1^−/− mice. Scale bar = 30 µm. Quantification of (D) LAMP1⁺, (E) Ubiquitin1⁺ and (F) nT-APP⁺ DNs in the cortex of 5xFAD;Cx3cr1^+/+ (black bars) and 5xFAD;Cx3cr1^−/− (grey bars) mice based on their size distribution at 4 months of age. < 500 µm = 50-500 µm, > 500 µm = 550-1000 µm. Data represents mean cortical DN abundance calculated using multiple sections from n = 6 mice (3 females, 3 males) of each genotype. Statistics done using two-way ANOVA (p.^int LAMP1, Ubiquitin-1 and nT-APP < 0.0001) with Tukey’s post-hoc tests.**p < 0.001, ***p < 0.0001, ****p < 0.00001, ns = not significant