Fig. 2

cPLA2 activity is greater in brains of ApoE4-TR mice compared with ApoE3-TR mice. The cortex of 8-month old ApoE3 and ApoE4-TR mice was collected to extract RNA and proteins. A cPLA2 mRNA level in the cortex was detected by qPCR. B Phosphorylated-cPLA2 and total cPLA2 protein levels in the cortex were detected by western blot. Densitometric quantification from B. D cPLA2 activity in mouse cortex homogenates were measured by cPLA2 activity assay kit. E LTB4 levels in mouse cortical homogenates were measured by an LTB4 assay kit. (n = 5 for each genotype for A-E, 3 males and 2 females). LTB4: leukotriene B4. Data are represented as mean ± SEM and analyzed by Student’s t-test (two-tailed). *p < 0.05