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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity

Fig. 7

Elevated GM2A reduces neurite integrity and neuronal firing of human neurons. A, B Western blot of GM2A and GAPDH (A) and quantification of GM2A levels (B) in lysates from human iNs overexpressing GM2A or control at MOI = 1, 3, or 5. N = 2 for each condition. C Neurite integrity of Day 21 human iNs overexpressing GM2A or control at MOI = 1, 3, or 5. N = 90 for each condition. Error bars represent mean  ± SEM. Statistical analyses used unpaired t-test. *, p < 0.05; ***, p < 0.001; ns, not significant. D Spontaneous firing rate of Day 21 human iNs overexpressing GM2A or control at MOI = 1, 3, or 5. N = 8 for each condition. Error bars represent mean  ± SEM. Statistical analyses used paired t-test. *, p < 0.05. E Representative images of Day 21 human iNs overexpressing control or GM2A at MOI = 5. Scale bars = 200 μm

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