Fig. 1
From: Trem2 deletion enhances tau dispersion and pathology through microglia exosomes
Trem2 deletion aggravates pathogenic dispersion of tau pathology from the entorhinal cortex to the hippocampus. A Schematic depicting AAV-P301L tau (AAV-tau) injection and analysis. B AAV particles expressing human tau (AAV-P301L tau, “AAV-tau”) were stereotactically injected into the medial entorhinal cortex (MEC) of wildtype (WT) or Trem2 KO mice at 4 months of age, and histological images from the MEC (upper panels) or hippocampal dentate gyrus (DG) (lower panels) were obtained by confocal microscopy 5 weeks post-injection. Representative images from the MEC were stained with AT8 (pS202, T205 tau) and AT180 (pT231 tau) antibodies (green), in addition to Iba1 (purple), and DAPI (blue) as indicated. Bar = 100 μm. C Quantification of AT8 and AT180-positive cells/mm2 in the MEC region or DG from WT and Trem2 KO brain (n = 8 animals/genotype); graphs represent mean ± SE. Statistical significance was determined using unpaired Student’s t-tests (****, p < 0.0001). D Representative histological images in MEC and DG brain regions stained for human tau (T13, green), Iba1 (purple) and nuclei (DAPI, blue) in WT and Trem2 KO mouse brain 5 weeks following AAV-tau injection. Bar = 50 μm. E Quantification of htau (T13)-positive cells/mm2 and number of Iba1-positive microglia in the MEC region or DG from WT and Trem2 KO brain (n = 8 animal/genotype); graphs represent mean ± SE. Statistical significance was determined using unpaired Student’s t-tests (****p < 0.0001)