Fig. 3
CD3 antibody immunostaining in young and aged mice with PBS or ɑ-syn pff injection. A CD3 immunostaining images of young mouse cohort at 1-month post injection. Images are from three different mice (1, 2 and 3) of three brain regions (M1/M2 or somatosensory cortex (Neocortex), basolateral and basomedial amygdala (Amygdala), and dorsal striatum (Striatum)). Left panels are PBS (vehicle) injected and right are ɑ-syn pff injected. B Image analysis of CD3 positive cell counts per 0.1mm2 of young mouse cohort at 1-month post injection. C CD3 immunostaining images of young mouse cohort at 3-months post injection. Same format as (A). D Image analysis of CD3 positive cell counts per 0.1mm2 of young mouse cohort at 3-months post injection. E CD3 immunostaining images of aged mouse cohort at 1-month post injection. Same format as (A). F Quantitative analysis of CD3 positive cell counts per 0.1mm2 of aged mouse cohort at 1-month post injection. G CD3 immunostaining images of aged mouse cohort at 3-months post injection. Same format as (A). H Image analysis of CD3 positive cell counts per 0.1mm2 of aged mouse cohort at 3-months post injection. I-K Comparison of image analysis of CD3 positive cell counts per 0.1 mm.2 in neocortex (I) amygdala (J) and striatum (K) of ɑ-syn pff injected mice at 1- and 3-months post-injection in young (blue) and aged (red) mouse cohorts. Scale bars, 40 μm. Data are mean ± SEM. Unpaired t test was used. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001