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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Aging exacerbates the brain inflammatory micro-environment contributing to α-synuclein pathology and functional deficits in a mouse model of DLB/PD

Fig. 6

Double immune-fluorescence analysis in young and aged mice with ɑ-syn pff injection. A Double immunofluorescence staining with phosphorylated ɑ-syn (p-ɑ-syn, 81A) and CD3 antibodies. Split and merged representative microscopy images from the somatosensory cortex, basolateral and basomedial amygdala, and dorsal striatum of ɑ-syn pff injected aged mouse double labeled with antibodies against p-ɑ-syn (green) and CD3 cells (T cells, red channel). B, C Quantitative analysis of distances between p-ɑ-syn and CD3 cells in neocortex, amygdala, and striatum in 1-month (B) or 3-months (C) post injection. D-F Comparison of image analysis of proximity between p-ɑ-syn and CD3 cells in neocortex, amygdala, and striatum in young and aged mouse cohorts. G Double immunofluorescence staining with Iba1 and CD3 antibodies. Split and merged representative microscopy images from the neocortex, amygdala, and striatum of ɑ-syn pff injected aged mouse double labeled with antibodies against p-ɑ-syn (green) and Iba1 (microglia cells, red channel). H, I Quantitative analysis of distances between p-ɑ-syn and microglia cells in neocortex, amygdala, and striatum in 1-month (H) or 3-months (I) post injection. J-L Comparison of image analysis of proximity between p-ɑ-syn and microglia cells in neocortex, amygdala, and striatum in young and aged mouse cohorts. Scale bars, 20 μm in low magnification, 10 μm in the high magnification. Data are mean ± SEM. Unpaired t test was used. * p < 0.05; ** p < 0.01; *** p < 0.001

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