Fig. 4

ApoE isoform-dependent transcriptional changes in mice upon cuprizone-induced demyelination. A An overview of genes with significant difference in fold change (FC) from CPZ vs control groups, among three apoE isoforms. B Volcano plot representation of gene FC among different apoE-TR mice treated with CPZ. The representative DEGs are shown. X-axis represents log2 fold change (CPZ vs CTL) and y-axis represents -log10 FDR adjusted P value. C Chart showing selected genes with significant fold changes identified through transcriptomics after CPZ treatment (E2 > E3 and E2 > E4). D Critical pathways enriched for the genes with significant FC difference. E Heat map showing main differentially regulated functional pathways. F Sunburst plot showing the MEGENA co-expression module hierarchy. Modules enriched for genes with significantly increases in FC in apoE2 compared to apoE4 are labeled. G Network topology of immune response module M245 is illustrated using Cytoscape. Node size is proportional to the network degree such that genes that harbor more relationships with other molecules have greater size. Labels are shown for nodes connected to at least 16 other nodes. Node color intensity denotes the differential expression t-statistics of comparing fold change between apoE2 and apoE4. Diamond shape denotes hub gene