Fig. 7

Differential effects of apoE isoforms on remyelination after cuprizone removal. ApoE-TR mice were fed with either normal diet (CTL) or CPZ diet for four weeks, following which CPZ diet was switched back to normal chow for two weeks to allow myelin recovery. A Brain samples of apoE-TR mice from different treatment groups (CTL, n = 9; CPZ and Recovery, n = 14–16/genotype) were subjected to immunofluorescence staining for MBP. Representative images of MBP⁺ (Green) staining in the CC region of apoE-TR mice are shown. Scale bar, 50 µm. B-D The immunoactivity of MBP was quantified. E MBP recovery (fold change of Recovery vs. CPZ) in CC area of apoE-TR mice was quantified. F Brain samples of apoE-TR mice from different groups (CTL, CPZ and Recovery, n = 4/genotype) were subjected to transmission electron microcopy (TEM) analysis. Representative images of myelinated axons in the CC region of apoE-TR mice are shown. Scale bar, 50 µm. G-I, Myelinated axons in the CC region of apoE-TR mice were quantified. J The extent of remyelination (fold change of Recovery vs. CPZ) in different apoE-TR mice was quantified. Values are mean ± SEM. One-way ANOVA. * P < 0.05; ** P < 0.01. N.S., not significant