Fig. 1
Microglia predominate within the corpus callosum following cuprizone induced demyelination. a Schematic of myeloid cells in CX3CR1creER;Rosa26tdTom mice 4 weeks post-tamoxifen. The tdTomato (tdTom) is restricted to microglia and border associated macrophages (BAM). b Representative immunohistographs of CX3CR1creER;Rosa26tdTom mice on a control, two, or four week, cuprizone diet demonstrating preferential microglia expansion (tdTom reporter, red; CD45, white). Quantification of the proportion (c) and density (d) of microglia/BAM (IBA1+ve/CD45+ve/tdTom+ve) and (e) monocyte-derived macrophages (IBA1 + ve/CD45+ve/tdTom-ve) show preferential expansion of microglia/BAM. f Schematic of myeloid cells in Tmem119creER;Rosa26tdTom mice. Quantification of the proportion (g) of microglia (IBA1+ve/tdTom+ve) and (h) representative images confirm preferential expansion of microglia after cuprizone. Arrows indicate tdTom-ve/CD45+ve cells. (c-e) n = 3 (control) or n = 6–8 (1,2,4 week after cuprizone); Ordinary one-way ANOVA with Tukey’s multiple comparisons test (p < 0.05). Error bar ± SEM. (b) Scale bar, 50 μm