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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Single-cell microglial transcriptomics during demyelination defines a microglial state required for lytic carcass clearance

Fig. 6

Microglia ablation causes accumulation of lytic carcasses. a Schematic of microglia ablation starting two weeks after mice began a cuprizone diet. b. Representative corpus callosum acridine orange (AO) stained section imaged after 10 days of diphtheria toxin treatment. Microglia ablation elevated lytic morphologies, based on morphological criteria to differentiate apoptotic morphologies (red arrowhead) and lytic morphologies (white arrowhead). This observation is reflected in quantification of total dead cell density (c) and cell density stratified by apoptotic and lytic morphologies (d). (e) Ten days of diphtheria toxin injections to ablate microglia did not increase the density of dead tdTomato-positive cells (microglia). f Representative AO-stained image overlaid with immunohistochemical image demonstrating lytic carcass inside of microglia. g In control mice fed cuprizone lytic carcasses were commonly found within microglia, but after microglia ablation, this phenomena was rare. (c, d, e, f) n = 5–6/group. (c, e) One-way ANOVA with Tukey’s multiple comparison test. d Two-way ANOVA with Holm-Sidak’s multiple comparisons test. g Welch’s T test. Error bar ± SEM. Scale bar, (b) 50 μm (f) 10 μm

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